Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Nucleotide sequence alignment of A. flavus (ATCC 16883), A. fumigatus (ATCC 36607), A. nidulans (ATCC 10074), A. niger (ATCC 16888), A. terreus (ATCC 16792), and A. ustus (ATCC 201953). The alignment consists of the 3′ end of the 18S ribosomal DNA (rDNA) gene (which contains the ITS 1 primer site), the complete ITS 1 region, the complete ITS 2 region, and the 5′ end of the 28S rDNA gene (which contains the ITS 4 primer site). The highly conserved 5.8S rDNA gene sequence has been omitted.

Article Snippet: The highly conserved 5.8S rDNA gene sequence has been omitted. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain % Similarity with strain: A. flavus ATCC 16883 A. fumigatus ATCC 36607 A. nidulans ATCC 10074 A. niger ATCC 16888 A. terreus ATCC 16792 sa A. ustus ATCC 201953 A. flavus ATCC 16883 A. fumigatus ATCC 36607 87.6 A. nidulans ATCC 10074 a 81.5 84.3 A. niger ATCC 16888 89.6 91.7 84.0 A. terreus ATCC 16792 87.0 91.1 83.0 90.6 A. ustus ATCC 201953 82.7 80.7 91.4 80.5 79.3 Open in a separate window a Accepted into GenBank as E. nidulans .

Techniques: Sequencing

Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Numbers of nucleotide differences in ITS 1–5.8S–ITS 2 within a single species

Article Snippet: The highly conserved 5.8S rDNA gene sequence has been omitted. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain % Similarity with strain: A. flavus ATCC 16883 A. fumigatus ATCC 36607 A. nidulans ATCC 10074 A. niger ATCC 16888 A. terreus ATCC 16792 sa A. ustus ATCC 201953 A. flavus ATCC 16883 A. fumigatus ATCC 36607 87.6 A. nidulans ATCC 10074 a 81.5 84.3 A. niger ATCC 16888 89.6 91.7 84.0 A. terreus ATCC 16792 87.0 91.1 83.0 90.6 A. ustus ATCC 201953 82.7 80.7 91.4 80.5 79.3 Open in a separate window a Accepted into GenBank as E. nidulans .

Techniques:

Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Aspergillus species PCR products

Article Snippet: The highly conserved 5.8S rDNA gene sequence has been omitted. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain % Similarity with strain: A. flavus ATCC 16883 A. fumigatus ATCC 36607 A. nidulans ATCC 10074 A. niger ATCC 16888 A. terreus ATCC 16792 sa A. ustus ATCC 201953 A. flavus ATCC 16883 A. fumigatus ATCC 36607 87.6 A. nidulans ATCC 10074 a 81.5 84.3 A. niger ATCC 16888 89.6 91.7 84.0 A. terreus ATCC 16792 87.0 91.1 83.0 90.6 A. ustus ATCC 201953 82.7 80.7 91.4 80.5 79.3 Open in a separate window a Accepted into GenBank as E. nidulans .

Techniques:

Journal: Metabolic Engineering Communications

Article Title: Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus

doi: 10.1016/j.mec.2022.e00203

Figure Lengend Snippet: Phylogenetic analysis of A. pseudoterreus strain ATCC32359. Calmodulin coding regions from selected Aspergillus species were downloaded from ENA and NCBI and aligned using Muscle. The consensus evolutionary history was inferred from 310 positions using the Maximum Likelihood method with 500 bootstrap replicates in MEGA .

Article Snippet: Genomic DNA and RNA was isolated from A. pseudoterreus (ATCC®32359TM) using a yeast genomic DNA purification kit (AMRESCO, Solon, OH) and Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI) respectively.

Techniques:

Journal: Metabolic Engineering Communications

Article Title: Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus

doi: 10.1016/j.mec.2022.e00203

Figure Lengend Snippet: Regulation of itaconic acid production by LaeA in Aspergillus pseudoterreus . Strains were cultivated in shake flasks for 8 days until all of the glucose and xylose present in the medium had been consumed from an initial concentration of 50 g/L total sugar. A) The major products of the cultivation are biomass and itaconic acid. Both are affected by laeA deletion and overexpression. B) Production of other tricarboxylic acid cycle derived organic acids at minor levels. Citric acid was not detected at > 5 mg/L in any sample. C) Correlation between itaconic acid production and final biomass or other acids. GX (glucose/xylose medium), P (phosphate), N (yeast extract). Asterisks indicate significant differences between either the laeA + or Δ laeA strain and the wild-type in each condition.

Article Snippet: Genomic DNA and RNA was isolated from A. pseudoterreus (ATCC®32359TM) using a yeast genomic DNA purification kit (AMRESCO, Solon, OH) and Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI) respectively.

Techniques: Concentration Assay, Over Expression, Derivative Assay

Journal: Metabolic Engineering Communications

Article Title: Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus

doi: 10.1016/j.mec.2022.e00203

Figure Lengend Snippet: Metabolic pathways that significantly change in response to LaeA overexpression. Genes with metabolic pathway annotations were predicted from the A. pseudoterreus genome. Metabolic pathways with at least 2 annotated genes that are enriched for significantly up- or down-regulated genes.

Article Snippet: Genomic DNA and RNA was isolated from A. pseudoterreus (ATCC®32359TM) using a yeast genomic DNA purification kit (AMRESCO, Solon, OH) and Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI) respectively.

Techniques: Over Expression, Starch

Journal: Metabolic Engineering Communications

Article Title: Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus

doi: 10.1016/j.mec.2022.e00203

Figure Lengend Snippet: Carbohydrate active enzymes that significantly change in response to LaeA overexpression. Carbohydrate-active enzymes were predicted from the A. pseudoterreus genome. Significantly up- and down-regulated genes ( q < 0.01) were identified and counted for each family.

Article Snippet: Genomic DNA and RNA was isolated from A. pseudoterreus (ATCC®32359TM) using a yeast genomic DNA purification kit (AMRESCO, Solon, OH) and Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI) respectively.

Techniques: Over Expression, Activity Assay, Binding Assay

Journal: Metabolic Engineering Communications

Article Title: Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus

doi: 10.1016/j.mec.2022.e00203

Figure Lengend Snippet: Genes involved in phosphate sensing and acquisition. A. pseudoterreus genes with homology to genes known to be involved in sensing and regulation of phosphate acquisition in Aspergillus nidulans , Neurospora crassa , and Saccharomyces cerevisiae were identified by BlastP. Delta values indicate the log 2 fold-change for the LaeA overexpression strain versus wild-type. Dashes indicate genes that were not detected at the transcript or protein level or for which insufficient data was available for statistical analysis.

Article Snippet: Genomic DNA and RNA was isolated from A. pseudoterreus (ATCC®32359TM) using a yeast genomic DNA purification kit (AMRESCO, Solon, OH) and Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI) respectively.

Techniques: Over Expression

Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Nucleotide sequence alignment of A. flavus (ATCC 16883), A. fumigatus (ATCC 36607), A. nidulans (ATCC 10074), A. niger (ATCC 16888), A. terreus (ATCC 16792), and A. ustus (ATCC 201953). The alignment consists of the 3′ end of the 18S ribosomal DNA (rDNA) gene (which contains the ITS 1 primer site), the complete ITS 1 region, the complete ITS 2 region, and the 5′ end of the 28S rDNA gene (which contains the ITS 4 primer site). The highly conserved 5.8S rDNA gene sequence has been omitted.

Article Snippet: Matrix of ITS 1–5.8S–ITS 2 similarities for referenced Aspergillus species table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain and accession no. No. of nucleotide base differences in: % Similarity ITS 1 ITS 2 ITS 1–5.8S–ITS 2 A. flavus ATCC 16883 IMI 210 0 1 1 99.8 AB008414 0 1 1 99.8 AB008415 0 2 2 99.7 AB008416 0 0 0 100.0 AF027863 0 0 0 100.0 AF078893 0 1 1 99.8 AF078894 0 0 0 100.0 L76747 4 0 4 99.3 A. fumigatus ATCC 36607 IMI 196 2 2 5 99.2 AF078889 2 0 2 99.7 AF078890 1 0 1 99.8 AF078891 1 0 1 99.8 AF078892 1 0 1 99.8 A. nidulans ATCC 10074 IMI 231 2 2 4 99.3 L76746 a 0 0 0 100.0 U03521 a NA c 2 2 99.6 A. niger ATCC 16888 IMI 026 0 0 0 100.0 AF078895 0 0 0 100.0 AJ223852 4 1 5 99.2 L76748 0 0 0 100.0 U65306 0 0 0 100.0 A. terreus ATCC 16792 IMI 203 4 0 4 99.3 AF078896 0 0 0 100.0 AF078897 0 0 0 100.0 AJ001334 0 0 0 100.0 AJ001335 0 0 0 100.0 AJ001338 0 0 0 100.0 AJ001368 0 1 1 99.8 L76774 0 0 0 100.0 U93684 0 3 3 99.5 A. ustus UAMH 9479 IMI 192 b 0 1 1 99.8 Open in a separate window a Deposited into GenBank as E. nidulans . b Deposited into the ATCC as ATCC 201953. c NA, not available.

Techniques: Sequencing

Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Numbers of nucleotide differences in ITS 1–5.8S–ITS 2 within a single species

Article Snippet: Matrix of ITS 1–5.8S–ITS 2 similarities for referenced Aspergillus species table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain and accession no. No. of nucleotide base differences in: % Similarity ITS 1 ITS 2 ITS 1–5.8S–ITS 2 A. flavus ATCC 16883 IMI 210 0 1 1 99.8 AB008414 0 1 1 99.8 AB008415 0 2 2 99.7 AB008416 0 0 0 100.0 AF027863 0 0 0 100.0 AF078893 0 1 1 99.8 AF078894 0 0 0 100.0 L76747 4 0 4 99.3 A. fumigatus ATCC 36607 IMI 196 2 2 5 99.2 AF078889 2 0 2 99.7 AF078890 1 0 1 99.8 AF078891 1 0 1 99.8 AF078892 1 0 1 99.8 A. nidulans ATCC 10074 IMI 231 2 2 4 99.3 L76746 a 0 0 0 100.0 U03521 a NA c 2 2 99.6 A. niger ATCC 16888 IMI 026 0 0 0 100.0 AF078895 0 0 0 100.0 AJ223852 4 1 5 99.2 L76748 0 0 0 100.0 U65306 0 0 0 100.0 A. terreus ATCC 16792 IMI 203 4 0 4 99.3 AF078896 0 0 0 100.0 AF078897 0 0 0 100.0 AJ001334 0 0 0 100.0 AJ001335 0 0 0 100.0 AJ001338 0 0 0 100.0 AJ001368 0 1 1 99.8 L76774 0 0 0 100.0 U93684 0 3 3 99.5 A. ustus UAMH 9479 IMI 192 b 0 1 1 99.8 Open in a separate window a Deposited into GenBank as E. nidulans . b Deposited into the ATCC as ATCC 201953. c NA, not available.

Techniques:

Journal:

Article Title: Identification of Aspergillus Species Using Internal Transcribed Spacer Regions 1 and 2

doi:

Figure Lengend Snippet: Aspergillus species PCR products

Article Snippet: Matrix of ITS 1–5.8S–ITS 2 similarities for referenced Aspergillus species table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain and accession no. No. of nucleotide base differences in: % Similarity ITS 1 ITS 2 ITS 1–5.8S–ITS 2 A. flavus ATCC 16883 IMI 210 0 1 1 99.8 AB008414 0 1 1 99.8 AB008415 0 2 2 99.7 AB008416 0 0 0 100.0 AF027863 0 0 0 100.0 AF078893 0 1 1 99.8 AF078894 0 0 0 100.0 L76747 4 0 4 99.3 A. fumigatus ATCC 36607 IMI 196 2 2 5 99.2 AF078889 2 0 2 99.7 AF078890 1 0 1 99.8 AF078891 1 0 1 99.8 AF078892 1 0 1 99.8 A. nidulans ATCC 10074 IMI 231 2 2 4 99.3 L76746 a 0 0 0 100.0 U03521 a NA c 2 2 99.6 A. niger ATCC 16888 IMI 026 0 0 0 100.0 AF078895 0 0 0 100.0 AJ223852 4 1 5 99.2 L76748 0 0 0 100.0 U65306 0 0 0 100.0 A. terreus ATCC 16792 IMI 203 4 0 4 99.3 AF078896 0 0 0 100.0 AF078897 0 0 0 100.0 AJ001334 0 0 0 100.0 AJ001335 0 0 0 100.0 AJ001338 0 0 0 100.0 AJ001368 0 1 1 99.8 L76774 0 0 0 100.0 U93684 0 3 3 99.5 A. ustus UAMH 9479 IMI 192 b 0 1 1 99.8 Open in a separate window a Deposited into GenBank as E. nidulans . b Deposited into the ATCC as ATCC 201953. c NA, not available.

Techniques: